Interleukin 5 (IL-5) BioAssay ELISA Kit (Canine)

£1,036.00 96 Tests

Interleukin 5 (IL-5) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL-5 in canine serum, plasma, tissue homogenates, cell lystaes, cell culture supernates and other biological fluids

Additional information

Detection Range

15.6-1000pg/ml

Sensitivity

5.9pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*026248A: Microtiter Strips, 1×48 wells or 1×96 wells, Pre-coated, ready to use
*026248B: Standard, 2×1 vial
026248C: Standard Diluent, 1x10ml or 1x20ml
*026248D: Detection Reagent A (green), 1x60ul or 1x120ul
*026248E: Detection Reagent B (red), 1x60ul or 1x120ul
026248F: Assay Diluent A, 1x6ml or 1x12ml
026248G: Assay Diluent B, 1x6ml or 1x12ml
026248H: TMB Substrate, 1×4.5ml or 1x9ml
*026248J: Positive Control, 185.6pg/ml, 1x1vial (Lyophilized)
026248K: Stop Solution, 1x3ml or 1x6ml
026248L: Wash Buffer, 30X, 1x10ml or 1x20ml

Precaution

The Stop Solution (026248K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *026248A, *026248B, *026248D, *026248E and *026248J at -20?C. After reconstitution of *026248J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 026248J

Positive Control

Intended Use

The positive control is from a canine serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The positive control vial contains canine IL-5 in canine serum (lyophilized powder). Note: The canine based serum used in this product are from healthy canine of SPF standard (specific pathogen-free animals).

Expected Values

185.6pg/ml

Usage

Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months