Interleukin 4 (IL4) BioAssay ELISA Kit (Rabbit)

£1,007.00 96 Tests

Interleukin 4 (IL4) BioAssay ELISA Kit (Rabbit) is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL4 in rabbit serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Additional information

Detection Range

15.6-1000pg/ml

Sensitivity

5.4pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*026235A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*026235B: Standard, 2×1 vial
026235C: Standard Diluent, 1x20ml
*026235D: Detection Reagent A, 1x120ul
*026235E: Detection Reagent B, 1x120ul
026235F: Assay Diluent A, 1x12ml
026235G: Assay Diluent B, 1x12ml
026235H: TMB Substrate, 1x9ml
*026235J: Positive Control, 194.6pg/ml, 1×1 vial
026235K: Stop Solution, 1x6ml
026235L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (026235K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *026235A, *026235B, *026235D, *026235E and *026235J at -20?C. After reconstitution of *026235J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 026235J

Positive Control

Intended Use

The control vial is from a rabbit serum sample known to contain IL4. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vial contains rabbit IL4 in rabbit serum (lyophilized powder). Note: The rabbit source material used in this product is from healthy rabbit of SPF standard (specific pathogen-free animals).

Expected Values

194.6pg/ml

Usage

Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Rabbit

Specificity

Rabbit

Shelf Life

6 Months