Interleukin 4 (IL4) BioAssay ELISA Kit (Canine)

£1,036.00 96 Tests

Interleukin 4 (IL4) BioAssay ELISA Kit (Canine) is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL4 in canine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Additional information

Detection Range

15.6-1000pg/ml

Sensitivity

5.8pg/ml

Precision

Intra-Assay:
CV<10%

Inter-Assay:
CV<12%

Kit Components

*026229A: Microtiter Plate, 1×48 wells or 1×96 wells
*026229B: Standard, 2x1vial
026229C: Standard Diluent, 1x10ml or 1x20ml
*026229D: Detection Reagent A (green), 1x60ul or 1x120ul
*026229E: Detection Reagent B (red), 1x60ul or 1x120ul
026229F: Assay Diluent A, 1x3ml or 1x6ml
026229G: Assay Diluent B, 1x3ml or 1x6ml
026229H: TMB Substrate, 1×4.5ml or 1x9ml
026229J: Positive Control, 466.4pg/ml, 1x1vial
026229K: Stop Solution, 1x3ml or 1x6ml
026229L: Wash Buffer, 30X, 1x10ml or 1x20ml

Precaution

The Stop Solution (026229K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *026229A, *026229B, *026229D, *026248E and *026229J at -20?C. After reconstitution of *026229J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Canine

Shelf Life

6 Months