Interleukin 13 (IL-13) BioAssay ELISA Kit (Human)

£1,406.00 1 Kit

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-13 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-13 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and colour develops in proportion to the amount of IL-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Additional information

Kit Components

I8435-01A: Microtiter Plate, 1×96 wells
I8435-01B: Pab (HRP), 1x21ml
I8435-01C: Standard*, 1x20ng
I8435-01D: Assay Diluent: 1x11ml
I8435-01E: (Cell Culture Supernatant), 1x21ml
I8435-01F: Calibrator Diluent (Serum/Plasma), 1x21ml
I8435-01G: Wash Buffer (25X), 1x21ml
I8435-01H: Color Reagent A (H2O2), 1×12.5ml
I8435-01J: Color Reagent B (TMB), 1×12.5ml
I8435-01K: Stop Solution, 1x6ml

Storage and Stability

Store all components at 4°C. Stable for 6 months. *Store I8435-01C at -20°C after reconstitution. Components are stable for 1 month at the appropriate temperature after opening/reconstitution.

Shipping Temperature

On Dry Ice

Source Antigen

Human

Shelf Life

6 Months