Interleukin 1 alpha (IL1a) BioAssay ELISA Kit (Rabbit)

£1,007.00 96 Tests

Interleukin 1 alpha (IL1a) BioAssay ELISA Kit (Rabbit) is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1a in rabbit serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Additional information

Detection Range

15.6-1000pg/ml

Sensitivity

5.7pg/ml

Precision

Intra-Assay:
CV<10%

Inter-Assay:
CV<12%

Kit Components

*026016A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*026016B: Standard, 2×1 vial
026016C: Standard Diluent, 1x20ml
*026016D: Detection Reagent A (green), 1x120ul
*026016E: Detection Reagent B (red), 1x120ul
026016F: Assay Diluent A, 1x12ml
026016G: Assay Diluent B, 1x12ml
026016H: TMB Substrate, 1x9ml
026016K: Stop Solution, 1x6ml
026016L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (026016K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *026016A, *026016B, *026016D and *026016E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Rabbit