Interleukin 1 alpha, Human, BioAssay ELISA Kit (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)

£943.00 96 Tests

This Human Interleukin-1a ELISA Kit is to be used for the in vitro quantitative determination of human interleukin-1a (IL-1a) concentrations in serum, plasma, cell culture supernatant, and other biological fluids

IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1a present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody-enzyme specific for IL-1a is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1a bound in the initial step. The color development is stopped and the intensity of the color is measured.

In order to measure the concentration of IL-1a in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-1a concentration (pg/ml). The concentration of IL-1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Additional information

Sensitivity

Calibrator Diluent I: 5.0pg/ml
Calibrator Diluent II: 4.6pg/ml

Range

15.6-500pg/ml

Kit Components

I7663-02B1:Microtiter Plate 1×96 wells
I7663-02B2: IL-1a (Biotin) Mab 1x11ml
I7663-02B3: Avidin (HRP) 1x14ml
*I7663-02B4: IL-1a Standard 2×1 vial
I7663-02B5: Calibrator Diluent I 1x22ml
I7663-02B6: Calibrator Diluent II 1x22ml .
I7663-02B7: Wash Buffer (20X) 1x60ml
I7663-02B8:. Substrate A 1x10ml
I7663-02B9: Substrate B 10ml
I7663-02B10: Stop Solution 1x14ml

Storage and Stability

Store at 4°C. Store I7663-02B4: IL-1a Standard powder at 4?C, liquid at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Temperature

On Dry Ice

Source Antigen

Human

Specificity

For the Quantitative Determination of Human Interleukin 1a
(IL-1a) Concentrations in Serum, Plasma, Cell Culture Supernatant, and Other Biological Fluids.

Shelf Life

6 Months