Interferon, gamma, Human, Low Range (IFNg) BioAssay ELISA Kit

£943.00 96 Tests

This Human IFN-g ELISA kit is to be used for the in vitro quantitative determination of concentrations in serum, plasma, cell culture supernatant and other biological fluids. It is intended for research use only and is not to be used in diagnostic or therapeutic procedures.

SKU: USI7662-16H Categories: ,

Additional information

Kit Components

I7662-16H1: FN-g Microtiter Plate 1×96 wells. Pre-coated with anti-human IFN-g monoclonal antibody.

I7662-16H2: Pab (HRP) 1x14ml. Anti-human IFN-g polyclonal antibody conjugated to horseradish peroxidase with preservative.

*I7662-16H3: IFN-g Standard 2x1vial. Recombinant human IFN-g (3.2ng/vial) in a buffered protein base with preservative, lyophilized.

I7662-16H4: Calibrator Diluent I 1x22ml. Animal serum with preservative. For serum/plasma testing.

I7662-16H5: Calibrator Diluent II (5X) 1x22ml. Cell culture medium with calf serum and preservative. For cell culture supernatant/urine testing.

I7662-16H6: Wash Buffer (20X) 1x60ml, 20-fold concentrated solution of buffered surfactant.

I7662-16H7: Substrate A 1x10ml
Buffered solution with H2O2.

I7662-16H8: Substrate B 1x10ml
Buffered solution with TMB.

I7662-16H9: Stop Solution 1x14ml
2N Sulphuric Acid (H2SO4). Caution: Caustic Material!

Sensitivity

I7662-16H4: Calibrator Diluent I 13.1pg/ml
I7662-16H5: Calibrator Diluent II 7.5pg/ml

Range

0-1600pg/ml

Storage and Stability

Store *I7662-16H3 powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Temperature

On Dry Ice

Source Antigen

Human

Specificity

This sandwich ELISA recognizes both natural and recombinant human IFN-g. This kit exhibits no detectable crossreactivity with human; SAA, EGF, IL-6, IL-8, IL-16, M-CSF, GM-CSF, TGF-b1, RANTES, FGF, MIP-1a, CRP, EPO.

Shelf Life

6 Months