Insulin Autoantibody (IAA) BioAssay ELISA Kit (Human)

£1,140.00 96 Tests

Insulin Autoantibody (IAA) BioAssay ELISA Kit (Human) is an enzyme immunoassay for in vitro quantitative measurement of IAA in human serum, plasma and other biological fluids.

SKU: US025830 Categories: , ,

Additional information

Detection Range

3.12-200ng/ml

Sensitivity

1.25ng/ml

Precision

Intra-Assay CV:
< 10%

Inter-Assay CV:
< 12%

Kit Components

*025830A: Microtiter Strip, 1×96 wells, Pre-coated, ready to use
*025830B: Standard, 2×1 vial
025830C: Standard Diluent, 1x20ml
*025830D: Detection Reagent, 1x120ul
025830F: Assay Diluent, 1x12ml
025830H: TMB Substrate, 1x9ml
025830K: Stop Solution, 1x6ml
025830L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (025830K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *025830A, *025830B, and *025830D at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent. Incubate 1 hour at 37?C.
4. Aspirate and wash 5 times.
5. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
6. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Specificity

Human

Shelf Life

6 Months