IgG1, Mouse, BioAssay ELISA Kit

£1,005.00 96 Tests

Test Principle:
The Mouse IgG1 ELISA kit is based on the binding of mouse IgG1 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgG1 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgG1 in samples and control is calculated from a curve of standards containing known concentrations of IgG1.

SKU: USI1904-72 Categories: , ,

Additional information

Intended Use

The IgG1 Murine BioAssay ELISA Kit is an in vitro immunoassay for the quantification of IgG1 circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.

Kit Components

I1904-72A: Microtiter Plate: 1×96 wells
I1904-72B: Positive Control (21-39ng/ml): 1x650ul
I1904-72C: Standard 5ng/ml, 1x650ul
I1904-72D: Standard 10ng/ml, 1x650ul
I1904-72E: Standard 20ng/ml, 1x650ul
I1904-72F: Standard 40ng/ml, 1x650ul
I1904-72G: Standard 100ng/ml, 1x650ul
I1904-72H: IgG1 (HRP), 100X, 1x150ul
I1904-72J: Sample Diluent, 20X, 1x10ml
I1904-72K: Wash Solution, 100X, 1x10ml
I1904-72L: TMB Substrate, 1x12ml
I1904-72M: Stop Solution, 1x12ml

Storage and Stability

Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Temperature

On Dry Ice

Source Antigen

Mouse

Shelf Life

6 Months