How do I use hPL?

  1. Thaw hPL overnight at 4°C or for 1 hour in a 37°C water bath.
  2. Prepare complete MSC culture medium by adding hPL to basal medium (i.e. Dulbecco’s Modified Eagles Medium-Low Glucose; DMEM-LG) with 2 mM L-glutamine and 100 U/mL Penicillin/streptomycin as final concentration.
  3. hPL shows optimal growth of MSC at 5% (v/v). However, for higher cell proliferation rates, it is recommended to use 10% (v/v) of hPL.
  4. We recommend seeding MSCs at approximately 3×103 ~ 6×103 per cm2.
  5. For Human Platelet Lysate Standard ONLY, to avoid coagulation of the complete MSC culture medium, Heparin should be added at a final concentration of 2 U/mL Heparin Standard (cat.no. PLA-001M) or 0.024 mg/mL Heparin Xeno-Free (cat. no. PLA-XF-001M).

NOTE: Human Platelet Lysate FD and Human Platelet Lysate XF DO NOT require the addition of heparin as they are fibrinogen depleted products.

  1. Complete MSC culture medium can be stored at 4°C and is stable for approximately four weeks.

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