Histamine (HIS) BioAssay ELISA Kit

£1,102.00 96 Tests

The Histamine (HIS) BioAssay ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of HIS in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Additional information

Detection Range

390.6-100,000pg/ml

Sensitivity

137.3pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*025678A: Microtiter Plate, 1×96 wells, Pre-coated, ready to use.
*025678B: Standard, 2x1vial
025678C: Standard Diluent, 1x20ml
*025678D: Detection Reagent A (green), 2x1vial
*025678E: Detection Reagent B (red), 1x120ul
025678F: Assay Diluent A, 1x12ml
025678G: Assay Diluent B, 1x12ml
025678H: TMB Substrate, 1x9ml
*025678J: Positive Control, 3820pg/ml, 1x1vial (Lyophilized)
025678K: Stop Solution, 1x6ml
025678L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (025678K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *025678A, *025678B, *025678D, *025678E and *025678J at -20?C. After reconstitution of *025678J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 025678J

Positive Control

Intended Use

The positive control is from human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The positive control vial contains human HIS in human serum (lyophilized powder). Note: The human based material used in this product is screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

Expected Values

3820pg/ml

Usage

Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well.
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months