Hepatitis B Virus E Antigen (HBeAg), ELISA Kit, BioAssay (Human)

£710.00 96 Tests

The Hepatitis B Virus E Antigen (HBeAg) ELISA Kit is a qualitative in vitro enzyme immunoassay for the detection of HBeAg in human serum or plasma.

Principle of the Assay:
Purified Anti-HBe is coated on the solid phase of multi-wells. Serum samples and Anti-HBe-HRP conjugate are added to coated wells. After incubation, if HBeAg is present in the sample, a complex of Anti-HBe-HBeAg-Anti-HBe-HRP will form. Wells are washed to remove other unbound serum components and incubated with substrate (TMB) to form a coloured product. The absorbance at 450nm is measured to indicate the presence or absence of HBeAg in the sample. The test is sensitive, reproducible and easy to operate.

SKU: USH1910-40K Categories: , ,

Additional information

Kit Components

H1910-40K1: Microtiter Plate, 1×96 wells. Coated with Anti-HBe
H1910-40K2: Enzyme Conjugate, 1×6.5ml
H1910-40K3: Positive Control Serum, 1x1ml
H1910-40K4: Negative Control Serum, 1x1ml
H1910-40K5: Wash Buffer, 20X, 1x50ml. Dilute 1:20 prior to use.
H1910-40K6: Substrate A, 1×8.5ml
H1910-40K7: Substrate B, 1×8.5ml
H1910-40K8: Stop Solution, 1×8.5ml

Storage and Stability

Store all kit components at 4°C. Kit is stable for 6 months after receipt.

Test Procedure

1. Set one blank, two positive controls and two negative controls for each assay. Add 50ul serum sample, positive control serum and negative control serum into the coated wells.

2. Add one drop (approximately 50ul) of Enzyme Conjugate into the wells, except the blank wells. Mix thoroughly, cover the wells with seal paper, and incubate for 30-60 minutes at 37?C.

3. Wash the wells.
Manual Wash Procedure: Remove the liquid completely from the coated wells. Fill the wells with wash solution and then remove the liquid completely. Repeat 5 times.
Automatic Wash Procedure: Select the automatic operation of washing to 5 times; ensure liquid has been removed completely after the last wash.

4. Add one drop (approximately 50ul) of Substrate A and B respectively to each well, seal the plate with seal paper, and incubate for 10-15 minutes at 37?C. Avoid exposure to light.

5. Add one drop (approximately 50ul) of Stop solution into each well and then mix thoroughly to terminate the reaction. Measure the absorbance at 450nm against the blank or measure the absorbance at 450nm/630-690nm.

Interpretation of Results

Cut-off Value (COV) Calculation:

COV = average OD450 of negative controls x 2.1

Positive: OD450 of sample ??COV

Negative: OD450 of sample < COV

Invalid: If the average OD of negative controls is ??0.1, the result is invalid. Repeat the test. If the problem persists, contact the local distributor.

Note: If the absorbance of negative controls is below 0.05, calculate it as 0.05. If the absorbance of negative controls is above 0.05, calculate it as its original value.

Sensitivity

2NCU/ml (per Biological Reference Reagents of Chinese Clinical Test Center), OD ? 1.500

Specificity

The average OD of 20 normal negative samples is ? 0.030

Precision

%CV ? 15% (n= 10)

Shipping Temperature

On Dry Ice

Source Antigen

Human

Grade

Molecular Biology Grade