Heme Oxygenase 1, Decycling (HO1) BioAssay ELISA Kit (Mouse)

£976.00 96 Tests

Heme Oxygenase 1, Decycling (HO1) BioAssay ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of HO1 in mouse serum, plasma, tissue homogenates, cell lysates and other biological fluids.

SKU: US025593 Categories: ,

Additional information

Detection Range

31.2-2000pg/ml

Sensitivity

13.8pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*025593A: Microtiter Strips, 1×48 wells or 1×96 wells, Pre-coated, ready to use
*025593B: Standard, 2×1 vial
025593C: Standard Diluent, 1x10ml or 1x20ml
*025593D: Detection Reagent A (green), 1x60ul or 1x120ul
*025593E: Detection Reagent B (red), 1x60ul or 1x120ul
025593F: Assay Diluent A, 1x6ml or 1x12ml
025593G: Assay Diluent B, 1x6ml or 1x12ml
025593H: TMB Substrate, 1×4.5ml or 1x9ml
*025593J: Positive Control, 401.9pg/ml, 1×1 vial
025593K: Stop Solution, 1x3ml or 1x6ml
025593L: Wash Buffer, 30X, 1x10ml or 1x20ml

Precaution

The Stop Solution (025593K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *025593A, *025593B, *025593D, *025593E and *025593J at -20?C. After reconstitution of *025593J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Mouse

Specificity

Mouse

Shelf Life

6 Months