Growth Hormone Releasing Hormone (GHRH) BioAssay ELISA Kit (Human)

£960.00 96 Tests

Growth Hormone Releasing Hormone (GHRH) BioAssay ELISA Kit (Human) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GHRH in human serum, plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants and Other Biological Fluids.

SKU: US025511 Categories: ,

Additional information

Detection Range

12.35-1000pg/ml

Sensitivity

≤6.15pg/ml

Precision

Intra-Assay:
CV <10%

Inter-Assay:
CV <12%

Kit Components

*025511A: Microtiter Plate, 1×96 wells, Pre-coated, ready to use
*025511B: Standard, 2x1vial?
025511C: Standard Diluent, 1x20ml
*025511D: Detection Reagent A, 1x120ul?
*025511E: Detection Reagent B, 1x120ul?
025511F: Assay Diluent A, 2x, 1x6ml
025511G: Assay Diluent B, 2x, 1x6ml
025511H: TMB Substrate, 1x9ml
025511K: Stop Solution, 1x6ml
025511L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (025511K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *025511A, *025511B, *025511D and *025511E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul working Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul working Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul 025511H: Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul 025511K: Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Specificity

Human

Shelf Life

6 Months