Ghrelin, Rat, Mouse, BioAssay ELISA Kit

£1,225.00 1 Kit

This Enzyme Immunoassay kit is designed to detect a specific peptide and its related peptides based on the principle of “competitive” enzyme immunoassay. Plasma, serum, culture media, tissue homogenate, CSF, urine or any biological fluid can be assayed as long as the level of the sample is high enough for the sensitivity of the kit to detect.

SKU: USG2033-73 Categories: , ,

Additional information

Plasma Extraction

Extraction is strongly recommended but not required.

Peptide Sequence Used in Kit

Gly-Ser-Ser(Octanoyl)-Phe-Leu-Ser-Pro-Glu-His-Gln-Lys-Ala-Gln-Gln-Arg-Lys-Glu-Ser-Lys-Lys-Pro-Pro-Ala-Lys-Leu-Gln-Pro-Arg

Kit Components

G2033-73A: 96-well Microplate1x1plate
G2033-73B: Assay Buffer (20X) 1x50ml
G2033-73C: Serum 1x5ml
G2033-73D: Standard1x1ug
G2033-73E: Peptide (Biotin) 1x5ml
G2033-73F: Streptavidin (HRP) 1x30ul
G2033-73G: Substrate solution 1x12ml
G2033-73H: Stop Solution, 2N HCI 1x15ml
G2033-73J: Positive Control 2x200ul

Sensitivity

Minimum Detectable Concentration: 0.1ng/ml

Precision

Intra-assay error: <5%
Inter-assay error: <14%

Range

0-100ng/ml

Linear Range

0.10-1.05ng/ml

Specificity

Peptide/% Crossreactivity
Ghrelin (Rat) 100
Ghrelin (Human)100
Ghrelin (Des-Octanoyl-Ser3) (Human)100
Ghrelin (Des-Octanoyl-Ser3) (Rat) 100
C-Terminal of Ghrelin 100
Ghrelin (Porcine) 0
Ghrelin (1-14) 0
Ghrelin (1-5) 0
Secretin (Human) 0
VIP (Human, Porcine, Rat) 0
Prolactin-Releasing Peptide-31 (Rat) 0
Prolactin-Releasing Peptide-31 (Human) 0
Galanin (Rat) 0
Galanin (Human) 0
GHRF (Rat) 0
GHRF (Human) 0
NPY (Human Rat) 0
Orexin A (Human Bovine Mouse Rat) 0
Orexin B (Human) 0
Orexin B (Mouse, Rat) 0
beta-Endorphin (Rat) 0
beta-Endorphin (Human) 0
alpha-MSH 0
MCH 0

Storage and Stability

Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Temperature

On Dry Ice

Source Antigen

Mouse

Shelf Life

6 Months