Ghrelin (GHRL) BioAssay ELISA Kit (Human)

£960.00 96 Tests

Ghrelin (GHRL) BioAssay ELISA Kit (Human) is a competitive inhibition enzyme immunoassay for in vitro quantitative measurement of GHRL in human serum, plasma, tissue homogenates, cell lysates, saliva, cell culture supernatants and other biological fluids.

SKU: US025266 Categories: , ,

Additional information

Detection Range

123.5-10,000pg/ml

Sensitivity

44.8pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*025266A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*025266B: Standard, 2x1vial
025266C: Standard Diluent, 1x20ml
*025266D: Detection Reagent A, 1x120ul
*025266E: Detection Reagent B, 1x120ul
025266F: Assay Diluent A, 1x12ml
025266G: Assay Diluent B, 1x12ml
025266H: TMB Substrate, 1x9ml
025266K: Stop Solution, 1x6ml
025266L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (025266K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *025266A, *025266B, *025266D, *025266E and *025266J at -20?C. After reconstitution of *025266J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well. And then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450 nm immediately.

Source Antigen

Human

Specificity

Human

Shelf Life

6 Months