Fibronectin (FN) BioAssay ELISA Kit (Mouse)

£976.00 96 Tests

Fibronectin (FN) BioAssay ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of FN in mouse serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Additional information

Detection Range

0.156-10ng/ml

Sensitivity

0.062ng/ml

Precision

Intra-Assay CV:
< 10%

Inter-Assay CV:
< 12%

Kit Components

*025102A: Microtiter Strip, 1×96 wells, Pre-coated, ready to use
*025102B: Standard, 2×1 vial
025102C: Standard Diluent, 1x20ml
*025102D: Detection Reagent A, 1x120ul
*025102E: Detection Reagent B, 1x120ul
025102F: Assay Diluent A, 1x12ml
025102G: Assay Diluent B, 1x12ml
025102H: TMB Substrate, 1x9ml
025102K: Stop Solution, 1x6ml
025102L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (025102K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *025102A, *025102B, *025102D and *025102E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Mouse

Specificity

Mouse

Shelf Life

6 Months