Fibroblast Growth Factor, Basic, Human (FGFb) BioAssay ELISA Kit

£1,121.00 96 Tests

This Human Fibroblast Growth Factor basic (FGF-b) ELISA kit is to be used for the quantitative determination of FGF basic in human serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18kD isoform and recombinant FGF basic-146 and FGF basic-157.

Principle of the Method:
This human FGF basic kit is a solid phase sandwich ELISA Assay. A monoclonal antibody specific for human FGF basic has been coated onto the wells of the microtiter strips provided. Samples, including standards of known human FGF-b content, control specimens, and unknowns, are pipetted into these wells. During the first incubation, the FGF-b antigen binds to the immobilized (capture) antibody on one site. After washing, a biotinylated monoclonal antibody specific for FGF-b is added. During the second incubation, this antibody binds to the immobilized FGF-b captured during the first incubation. After removal of excess second antibody, Streptavidin-Peroxidase (enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce colour. The intensity of this coloured product is directly proportional to the concentration of FGF-b present in the original specimen.

SKU: USF4210-19 Categories: ,

Additional information

Sensitivity

<15.6pg/ml

Range

15.6-1000pg/ml

Incubation Time

4 hours, RT

Simple

Pre-coated strip-well plates. Liquid stable conjugate, chromogen and stop reagents.

Sample Size

50ul

Accuracy

Inter-assay CV: <6.7%
Intra-assay CV: <5.0%

Kit Components

F4210-19A: Microtiter Plate, 1x96wells. FGFb antibody-coated wells.
F4210-19B: Standard 2x1vial.
F4210-19C: Standard Diluent, 1x25ml. Contains 0.1% sodium azide.
F4210-19D: FGFb (Biotin) Pab, 1x11ml. Contains 0.1% sodium azide.
F4210-19E: Streptavidin (HRP), 100X, 1x125ul. Contains 3.3mM thymol.
F4210-19F: Streptavidin-HRP Diluent, 1x25ml. Contains 3.3mM thymol.
F4210-19G: Wash Buffer Concentrate, 25X, 1x100ml.
F4210-19H: TMB, 1x25ml.
F4210-19J: Stop Solution, 1x25ml.

Storage and Stability

Store all components at 4°C. Stable for at 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Temperature

On Dry Ice

Source Antigen

Human

Shelf Life

6 Months