Fibrinogen Like Protein 2 (FGL2) BioAssay ELISA Kit (Mouse)

£976.00 96 Tests

This Fibrinogen Like Protein 2 (FGL2) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FGL2 in mouse plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

SKU: US025017 Categories: ,

Additional information

Detection Range

0.312-20ng/ml

Sensitivity

0.135ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*025017A: Microtiter Strips, 1×96 wells
*025017B:. Standard, 2×1 vial
025017C: Standard Diluent, 1x20ml
*025017D: Detection Reagent A (green), 1x120ul
*025017E: Detection Reagent B (red), 1x120ul
025017F: Assay Diluent A, 1x12ml
025017G: Assay Diluent B, 1x12ml
025017H: TMB Substrate, 1x9ml
*025017J: Positive Control, 4.32ng/ml, 1x1vial (Lyophilized)
025017K: Stop Solution, 1x6ml
025017L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (025017K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *025017A, *025017B, *025017D, *025017E and *025017J at -20?C. After reconstitution of *025017J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 025017J

Positive Control

Intended Use

The control vials are from a mouse plasma sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Charactersitics

The control vial contain mouse FGL2 in mouse plasma (lyophilized powder). Note: The mouse based serum used in this product are from healthy mouse of SPF standard (specific pathogen-free animals).

Expected Value

4.32ng/ml

Usage

Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months