Fetuin, Human, BioAssay ELISA Kit (alpha-2 Heremans-Schmid Glycoprotein, FHSG, Fetuin A)

£1,051.00 1 Kit

Human Fetuin-A ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Fetuin-A protein in serum. It is intended for in vitro research use only.

Features:

  • The total assay time is less than 3 hours.
  • The kit measures serum Fetuin-A/AHSG.
  • Standard is human plasma based.
  • Quality Controls are human serum based.
  • Components in the kit are provided ready-to-use, concentrated and lyophilized.
SKU: USF4102-01 Categories: , ,

Additional information

Kit Components

F4102-01A: Microtiter Strips, 1×96 wells,
F4102-01B: Pab (HRP), 1x13ml
*F4102-01C: Master Calibrator, 1x100ng
F4102-01D: High Control, 2x1vial
F4102-01E: Low Control, 2x1vial
F4102-01F: Dilution Buffer, 10x, 1x20ml
F4102-01G: Wash Solution, 10x, 1x100ml
F4102-01H: Substrate Solution (TMB), 1x13ml
F4102-01J: Stop Solution, H2SO4, 1x13ml

Sensitivity

Limit of Detection (LOD), defined as concentration of analyte giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of the blank: Ablank + 3xSDblank, is calculated from real fetuin-A values in wells is 0.35ng/ml. *Dilution buffer is pipetted into blank wells.

Specificity

Highly specific for human Fetuin-A protein. Crossreactivity was observed with monkey. No crossreactivity with serum of other animal species (bovine, feline, canine, hamster, goat, equine, mouse, porcine, rabbit, rat, sheep) has been observed.

Storage and Stability

Store at 4°C. Stable for 6 months at 4°C.
*Note: Store F4102-01C at 4°C as a lyophilized powder. Upon reconstitution, aliquot and store at -20°C. Stable for 6 months at -20?C.

Source Antigen

Human

Shelf LIfe

6 Months