Erythropoietin (EPO) BioAssay ELISA Kit (Rhesus Monkey)

£1,113.00 96 Tests

The Rhesus Monkey (Simian) Erythropoietin (EPO) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of EPO in simian serum, plasma and other biological fluids.

SKU: US024882 Categories: ,

Additional information

Detection Range

46.88-3000pg/ml

Sensitivity

17.25pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Test Principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to EPO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for EPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of EPO in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Kit Components

*024882A: Microtiter Strip, 1×96 wells, Pre-coated; ready to use
*024882B: Standard, 2x1vial
024882C: Standard Diluent, 1x20ml
*024882D: Detection Reagent A, 1x120ul
*024882E: Detection Reagent B, 1x120ul
024882F: Assay Diluent A, 1x12ml
024882G: Assay Diluent B, 1x12ml
024882H: TMB Substrate, 1x9ml
*024882J: Positive Control, 200.5pg/ml, 1×1 vial
024882K: Stop Solution, 1x6ml
024882L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (024882K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024882A, *024882B, *024882D, *024882E and *024882J at -20?C. After reconstitution of *024882J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Rhesus Monkey

Specificity

Rhesus Monkey

Shelf Life

6 Months