Endostatin (ES) BioAssay ELISA Kit (Mouse)

£976.00 96 Tests

Endostatin (ES) BioAssay ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ES in mouse serum, plasma and other biological fluids.

SKU: US024795 Categories: , ,

Additional information

Detection Range

0.156-10ng/ml

Sensitivity

0.056ng/ml

Precision

Intra-Assay CV:
< 10%

Inter-Assay CV:
< 12%

Kit Components

*024795A: Microtiter Strip, 1×96 wells, Pre-coated, ready to use
*024795B: Standard, 2×1 vial
024795C: Standard Diluent, 1x20ml
*024795D: Detection Reagent A, 1x120ul
*024795E: Detection Reagent B, 1x120ul
024795F: Assay Diluent A, 1x12ml
024795G: Assay Diluent B, 1x12ml
024795H: TMB Substrate, 1x9ml
*024795J: Positive Control, 1x1vial
024795K: Stop Solution, 1x6ml
024795L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (024795K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024795A, *024795B, *024795D, *024795E and *024795J at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Mouse

Specificity

Mouse

Shelf Life

6 Months