D-Dimer (D2D) BioAssay ELISA Kit (Porcine)

£1,068.00 96 Tests

D-Dimer (D2D) BioAssay ELISA Kit (Porcine) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of D2D in porcine plasma.

Additional information

Detection Range

61.7-5000ng/ml

Sensitivity

23.9ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Test Principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for D2D has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled D2D and unlabeled D2D (standards or samples) with the pre-coated antibody specific for D2D. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of D2D in the sample. After addition of the substrate solution, the intensity of the color developed is inversely proportional to the concentration of D2D in the sample.

Kit Components

*024575A: Microtiter Strips, 1×48 wells or 1x96wells
*024575B: Standard, 2×1 vial
024575C: Standard Diluent, 1x20ml
*024575D: Detection Reagent A (green), 1x1vial
*024575E: Detection Reagent B (red), 1x120ul
024575F: Assay Diluent A, 1x12ml
024575G: Assay Diluent B, 1x12ml
024575H: TMB Substrate, 1x9ml
024575J: Positive control, 613.7ng/ml, 1x1vial
024575K: Stop Solution, 1x6ml
024575L: Wash Buffer, 30X, 1x20ml
024575M: Detection Diluent Reagent A for 024575D, 1x300ul

Precaution

The Stop Solution (024575K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024575A, *024575B, *024575D, *024575E and *024575J at -20?C. After reconstitution of *024575J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 024575J

Positive Control

Intended Use

The control vials are from a porcine plasma sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vials contain porcine D2D from porcine plasma (lyophilized powder). Note: The porcine based plasma used in this product is from a healthy specimen(s) of SPF standard (specific pathogen-free animals).

Expected Values

613.7ng/ml

Usage

Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Porcine

Shelf Life

6 Months