Cytochrome c BioAssay ELISA Kit

£1,291.00 1 Kit

Principle of the Assay:
The Cytochrome C ELISA kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody specific for Cytochrome c has been coated onto the wells of the microtiter strips provided. Samples, including a standard containing Cytochrome c, control specimens and unknowns, are pipetted into these wells. During the first incubation, the Cytochrome c antigen binds to the immobilized (capture) antibody. After washing, a biotinylated secondary antibody specific for Cytochrome c is added to the wells. During the second incubation, this antibody serves as a detection antibody by binding to the immobilized Cytochrome c protein captured during the first incubation. After removal of excess detection antibody, a Streptavidin-Peroxidase (enzyme) is added. This binds to the biotinylated detection antibody to complete the four-member sandwich. After a third incubation and washing to remove all the excess Streptavidin-Peroxidase, a substrate solution is added, which is acted upon by the bound enzyme to produce colour. The intensity of this coloured product is directly proportional to the concentration of Cytochrome c present in the original specimen.

SKU: USC9094-90 Categories: , ,

Additional information

Kit Components

C9094-90A: Microwell Plate, 1×96 wells Supplied as removable strips coated with mouse monoclonal antibody to Cytochrome c.

C9094-90B*: Standard, 2 x1vial Supplied as 10ng of lyophilized powder.

C9094-90C: Standard Diluent Buffer, 1x25ml Supplied as a liquid, 0.1% sodum azide, red dye**.

C9094-90D: Pab (Biotin), 1x11ml Biotin-labeled anti-Cytochrome c antibody. Supplied as a liquid, 0.1% sodium azide, blue dye**.

C9094-90E: Streptavidin (HRP) 100X, 1x125ul Supplied as a liquid, 3.3mM thymol, 50% glycerol.

C9094-90F: HRP Diluent, 1x25ml Supplied as a liquid, 3.3mM thymol, yellow dye**.

C9094-90G: Wash Buffer 25X, 1x100ml

C9094-90H: TMB Substrate, 1x25ml

C9094-90J: Stop Solution, 1x25ml

Storage and Stability

*Store reconstituted C9094-90B at -70°C. Store all other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Analytical Sensitivity

<0.156ng/ml of Cytochrome c

Specificity

Detects human, rat and mouse forms of the Cytochrome c protein.

Shipping Temperature

On Dry Ice

Shelf Life

6 Months