Cross Linked N-Telopeptide of Type I Collagen (NTXI) BioAssay ELISA Kit (Rat)

£1,007.00 96 Tests

Cross Linked N-Telopeptide of Type I Collagen (NTXI) BioAssay ELISA Kit (Rat) is a competitive inhibition immunoassay for in vitro quantitative measurement of NTXI in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Additional information

Detection Range

2.47-200ng/ml

Sensitivity

0.92ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*024474A: Microtiter Strips, 1×96 wells
*024474B: Standard, 2×1 vial
024474C: Standard Diluent, 1x20ml
*024474D: Detection Reagent A (green), 1x120ul
*024474E: Detection Reagent B (red), 1x120ul
024474F: Assay Diluent A, 1x12ml
024474G: Assay Diluent B, 1x12ml
024474H: TMB Substrate, 1x9ml
024474K: Stop Solution, 1x6ml
024474L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (024474K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024474A, *024474B, *024474D and *024474E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Rat

Shelf Life

6 Months