Cross Linked C-Telopeptide of Type III Collagen (CTXIII) BioAssay ELISA Kit (Human)

£960.00 96 Tests

The Cross Linked C-Telopeptide of Type III Collagen (CTXIII) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of CTXIII in human serum, plasma and other biological fluids.

Additional information

Detection Range

123.5-10,000pg/ml

Sensitivity

46.8pg/ml

Precision

Intra-Assay CV: <10%
Inter-Assay CV: <12%

Kit Components

*024471A: Microtiter Plate, 1×48 wells or 1×96 wells, Pre-coated, ready to use.
*024471B: Standard, 2x1vial
024471C: Standard Diluent, 1x10ml or 1x20ml
*024471D: Detection Reagent A, 1x1vial
*024471E: Detection Reagent B, 1x60ul or 1x120ul
024471F: Assay Diluent A, 1x6ml or 1x12ml
024471G: Assay Diluent B, 1x6ml or 1x12ml
024471H: TMB Substrate, 1×4.5ml or 1x9ml
024471K: Stop Solution, 1x3ml or 1x6ml
024471L: Wash Buffer, 30X, 1x10ml or 1x20ml
024471M: Detection Reagent A Diluent for 024471D, 1x300ul

Precaution

The Stop Solution (024471K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024471A, *024471B, *024471D and *024471E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.

Shelf Life

6 Months