Cross Linked C-Telopeptide of Type II Collagen (CTXII) BioAssay ELISA Kit (Rat)

£1,007.00 96 Tests

Cross Linked C-Telopeptide of Type II Collagen (CTXII) BioAssay ELISA Kit is a competitive inhibition immunoassay for in vitro quantitative measurement of CTXII in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Additional information

Detection Range

98.8.5-8000pg/ml

Sensitivity

34.7pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*024470A: Microtiter Strips, 1×48 wells or 1×96 wells, Pre-coated, ready to use
*024470B: Standard, 2×1 vial
024470C: Standard Diluent, 1x10ml or 1x20ml
*024470D: Detection Reagent A, 1x1vial
*024470E: Detection Reagent B, 1x60ul or 1x120ul
024470F: Assay Diluent A, 1x6ml or 1x12ml
024470G: Assay Diluent B, 1x6ml or 1x12ml
024470H: TMB Substrate, 1×4.5ml or 1x9ml
*024470J: Positive Control, 208.6pg/ml, 1x1vial
024470K: Stop Solution, 1x3ml or 1x6ml
024470L: Wash Buffer, 30X, 1x10ml or 1x20ml
024470M: Detection Reagent A Diluent for 024470D, 1x300ul

Precaution

The Stop Solution (024470K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024470A, *024470B, *024470D, *024470E and *024470J at -20?C. After reconstitution of *024470J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 024470J

Positive Control

Intended Use

The positive control is from rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vial contains rat CTX11 in rat serum (lyophilized powder).
Note: The mouse based serum used in this product are from healthy mouse of SPF standard (Specific Pathogen-Free animals).

Expected Value

208.6pg/ml

Usage

Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Rat

Shelf Life

6 Months