Corticotropin Releasing Hormone (CRH) BioAssay ELISA Kit (Human)

£960.00 96 Tests

Corticotropin Releasing Hormone (CRH) BioAssay ELISA Kit (Human) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of CRH in human serum, plasma and other biological fluids.

SKU: US024434 Categories: ,

Additional information

Detection Range

12.35-1000pg/ml

Sensitivity

4.83pg/ml

Precision

Intra-Assay CV:
< 10%

Inter-Assay CV:
< 12%

Kit Components

*024434A: Microtiter Strips, 1×96 wells *024434B: Standard, 2×1 vial
024434C: Standard Diluent, 1x20ml
*024434D: Detection Reagent A (green), 1x120ul *024434E: Detection Reagent B (red) 1x120ul
024434F: Assay Diluent A, 1x12ml 1
024434G: Assay Diluent B, 1x12ml
024434H: TMB Substrate, 1x9ml
024434K: Stop Solution, 1x6ml
024434L: Wash Buffer, 30X, 1x20ml
024434M: Detection Reagent A Diluent, 1x300ul

Precaution

The Stop Solution (024434K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing
protection when using this material.

Storage and Stability

Store *024434A, *024434B, *024434D and *024434E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Shelf Life

6 Months