Connective Tissue Growth Factor (CTGF) BioAssay ELISA Kit (Rat)

£1,007.00 96 Tests

Connective Tissue Growth Factor (CTGF) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CTGF in rat serum, plasma, tissue homogenates, cell lysates, cell culture superantes and other biological fluids.

SKU: US024398 Categories: , ,

Additional information

Detection Range

31.2-2000pg/ml

Sensitivity

13.1pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*024398A: Microtiter Plate, 1×96 wells, Pre-coated; ready to use
*024398B: Standard, 2×1 vial
024398C: Standard Diluent, 1x20ml
*024398D: Detection Reagent A (green), 1x120ul
*024398E: Detection Reagent B (red), 1x120ul
024398F: Assay Diluent A, 1x12ml
024398G: Assay Diluent B, 1x12ml
024398H: TMB Substrate, 1x9ml
*024398J: Positive Control, 278.6pg/ml, 1x1vial (Lyophilized)
024398K: Stop Solution, 1x6ml
024398L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (024398K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024398A, *024398B, *024398D, *024398E and *024398J at -20?C. After reconstitution of *024398J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 024398J

Positive Control

Intended Use

The control vial is from a rat serum sample known to contain CTGF. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vial contains rat CTGF in rat serum (lyophilized powder). Note: The rat source material used in this product is from healthy rat of SPF standards (specific pathogen-free animals).

Expected Values

278.6pg/ml

Usage

Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens.
Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour a 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months