Connective Tissue Growth Factor (CTGF) BioAssay ELISA Kit (Mouse)

£976.00 96 Tests

Connective Tissue Growth Factor (CTGF) BioAssay ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CTGF in mouse serum, plasma, tissue homogenates and other biological fluids.

SKU: US024399 Categories: , ,

Additional information

Detection Range

62.5-4000pg/ml

Sensitivity

29.6pg/ml

Precision

Intra-Assay:
CV <10%

Inter-Assay:
CV <12%

Kit Components

*024399A: Microtiter Plate, 1×96 wells, Pre-coated, ready to use
*024399B: Standard, 2×1 vial?
024399C: Standard Diluent, 1x20ml
*024399D: Detection Reagent A (100X), 1x120ul?
*024399E: Detection Reagent B (100X), 1x120ul?
024399F: Assay Diluent A (2X), 1x12ml
024399G: Assay Diluent B (2X), 1x12ml
024399H: TMB Substrate, 1x9ml
024399K: Stop Solution, 1x6ml
024399L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (024399K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024399A, *024399B, *024399D and *024399E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Add 100ul working Detection Reagent A immediately. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul working Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Mouse

Shelf Life

6 Months