Complement Factor I (CFI) BioAssay ELISA Kit (Human)

£990.00 96 Tests

Complement Factor I (CFI) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CFI in human serum, plasma and other biological fluids.

SKU: US024389 Categories: , ,

Additional information

Detection Range

0.29-18ng/ml

Sensitivity

0.13ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*024389A: Microtiter Strips, 1×96 wells
*024389B: Standard, 2×1 vial
024389C: Standard Diluent, 1x20ml
*024389D: Detection Reagent A, 1x120ul
*024389E: Detection Reagent B, 1x120ul
024389F: Assay Diluent A, 1x12ml
024389G: Assay Diluent B, 1x12ml
024389H: TMB Substrate, 1x9ml
*024389J: Positive Control, 1.76ng/ml, 1×1 vial
024389K: Stop Solution, 1x6ml
024389L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (024389K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024389A, *024389B, *024389D, *024389E and *024389J at -20?C. After reconstitution of *024389J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 024389J

Positive Control

Intended Use

The control vial is from a human serum sample known to contain CFI. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vial contains human CFI in human serum (lyophilized powder). Note: The human source material used in this product is screened for HIV, Hepatits A, B, C, HTLV and Syphilis.

Expected Values

1.76ng/ml

Usage

Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Source Antigen

Human

Shelf Life

6 Months