Complement Component 5a (C5a) BioAssay ELISA Kit (Mouse)

£915.00 96 Tests

Complement Component 5a (C5a) BioAssay ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of C5a in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US024362 Categories: , ,

Additional information

Detection Range

78-5000pg/ml

Sensitivity

28pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*024362A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*024362B: Standard, 2×1 vial
024362C: Standard Diluent, 1x20ml
*024362D: Detection Reagent A (green), 1x120ul
*024362E: Detection Reagent B (red), 1x120ul
024362F: Assay Diluent A, 1x12ml
024362G: Assay Diluent B, 1x12ml
024362H: TMB Substrate, 1x9ml
*024362J: Positive Control, 1240.3pg/ml, 1×1 vial
024362K: Stop Solution, 1x6ml
024362L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (024362K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024362A, *024362B, *024362D, *024362E and *024362J at -20?C. After reconstitution of *024362J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Mouse

Shelf Life

6 Months