Collagen Type IV Alpha 1 (COL4a1) BioAssay ELISA Kit (Human)

£962.00 96 Tests

The Human Collagen Type IV alpha 1 (COL4a1) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of COL4a1 human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Additional information

Detection Range

4.7-300ng/ml

Sensitivity

2.1ng/ml

Precision

Intra-Assay:
CV<10%

Inter-Assay:
CV<12%

Kit Components

*024287A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*024287B: Standard, 2x1vial
024287C: Standard Diluent, 1x20ml
*024287D: Detection Reagent A (green), 1x120ul
*024287E: Detection Reagent B (red), 1x120ul
024287F: Assay Diluent A, 1x12ml
024287G: Assay Diluent B, 1x12ml
024287H: TMB Substrate, 1x9ml
*024287J: Positive Control, 16.8ng/ml, 1x1vial (Lyophilized)
024287K: Stop Solution, 1x6ml
024287L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (024287K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024287A, *024287B, *024287D, *024287E and *024287J at -20?C. After reconstitution of *024287J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 024287J

Positive Control

Intended Use

The control vial is from a human serum sample known to contain COL4a1. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vial contains human COL4a1 in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

Expected Values

16.8ng/ml

Usage

Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens.
Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months