Coagulation Factor VIII (F8) BioAssay ELISA Kit (Mouse)

£1,007.00 96 Tests

The Coagulation Factor VIII (F8) BioAssay ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of F8 in mouse serum, plasma and other biological fluids.

SKU: US024234 Categories: ,

Additional information

Detection Range

6.25-400pg/ml

Precision

Intra-Assay CV:
< 10%

Inter-Assay CV:
< 12%

Sensitivity

2.44pg/ml

Test Principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to F8. Standards and samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for F8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of F8 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Kit Components

*024234A: Microtiter Plate, 1x 96 wells. Pre-coated, ready to use
*024234B: Standard, 2×1 vial
024234C: Standard Diluent, 1x20ml
*024234D: Detection Reagent A, 1x120ul
*024234E: Detection Reagent B, 1x120ul
024234F: Assay Diluent A, 2X, 1x6ml
024234G: Assay Diluent B, 2X, 1x6ml
024234H: TMB Substrate, 1x9ml
024234K: Stop Solution, 1x6ml
024234L: Wash Buffer, 30X, 1x20ml

Storage and Stability

Store *024234A, *024234B, *024234D and *024234E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Source Antigen

Mouse

Specificity

Mouse

Shelf Life

6 Months