Ceruloplasmin (CP) BioAssay ELISA Kit (Human)

£901.00 96 Tests

Ceruloplasmin (CP) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US024062 Categories: , ,

Additional information

Detection Range

15.6-1000ng/ml

Sensitivity

7.4ng/ml

Precision

Intra-Assay:
CV <10%

Inter-Assay:
CV <12%

Kit Components

*024062A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*024062B: Standard, 2×1 vial
024062C: Standard Diluent, 1x20ml
*024062D: Detection Reagent A, 1x120ul
*024062E: Detection Reagent B, 1x120ul
024062F: Assay Diluent A, 1x12ml
024062G: Assay Diluent B, 1x12ml
024062H: TMB Substrate, 1x9ml
*024062J: Positive Control, 192.8ng/ml, 1×1 vial
024062K: Stop Solution, 1x6ml
024062L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (024062K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *024062A, *024062B, *024062D, *024062E and *024062J at -20?C. After reconstitution of *024062J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Notes on 024062J

Positive Control

Intended Use

The control vial is from a human serum sample known to contain CP. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vial contains human CP in human serum (lyophilized powder). Note: The human source material used in this product is screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

Expected Values

192.8ng/ml

Usage

Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Source Antigen

Human

Shelf Life

6 Months