CELLSPLIT®

An Innovative Xeno-free Cell Dissociation Media

CellSplit® is a completely xeno-free, direct replacement to porcine or bovine-derived trypsin. It has been developed so that you can use the same protocol, eliminating any disruption to your current procedure.

Benefits:

        • Completely xeno-free
        • Not derived from bacterial cultures
        • Plant-derived enzyme manufactured to GMP
        • UK CA and CE marked
        • Simple protocol
        • Inactivation by dilution, no need to use inactivation reagents such as FBS
  • CellSplit® is suitable for both serum-free and serum-containing cell culture protocols due to the inactivation by dilution. Unlike trypsin, there is no requirement for specific inhibitors, such as foetal bovine serum (FBS).

    The active ingredient is of plant origin and manufactured to GMP grade. A cysteine protease that has broad specificity, cleaving the peptide bonds of leucine and glycine. Cysteine proteases have been extensively used for cell and tissue disassociation.

    CellSplit® has been comprehensively tested with a wide range of cell lines including Vero, HEK, C2, C12, L929, MRC5, LLCPK1, MDCK and CHO. It has also been tested with primary canine stem cells (see CellSplit® data).

    To find out more and discuss your specific needs please contact sales@lifesciencegroup.co.uk, call +44 (0) 1234 889180 or complete the enquiry form.

    Dissociation times for cell lines:

    Figure 3.1.4: Comparison of cell dissociation percentage rates over time between reagents on Vero cell lines. Figure shows the increased rate of the dissociation of Vero cells incubated in Cellsplit (blue) In comparison to its competitors TrypLE (green), Trypsin (red). Data shown as means (two-way ANOVA test) ****p ≤ 0.0001. 

    Figure 3.1.5: Comparison of cell dissociation percentage rates over time between reagents on MRC-5 cell lines. Figure shows the increased rate of the dissociation of MRC-5 cells incubated in Cellsplit (blue) In comparison to its competitors TrypLE (green), Trypsin (red). Data shown as means (two-way ANOVA test) ****p ≤ 0.0001. 

    Dissociation times of three primary canine mesenchymal stem cell lines:

    Comparison of cell dissociation percentage rates over time between reagents on CMSC 2116 cell lines (canine MSC). Figure shows the increased rate of the dissociation of CMSC 2116 cells incubated in Cellsplit (blue) In comparison to its competitors TrypLE (green), Trypsin (red).  Data shown as means (two-way ANOVA test) ****p ≤ 0.0001.  

    Figure 3.1.2: Comparison of cell dissociation percentage rates over time between reagents on CMSC 2118 cell lines (canine MSC). Figure shows the increased rate of the dissociation of CMSC 2118 cells incubated in Cellsplit (blue) In comparison to its competitors TrypLE (green), Trypsin (red). Data shown as means (two-way ANOVA test) ****p ≤ 0.0001.

    Figure 3.1.3: Comparison of cell dissociation percentage rates over time between reagents on CMSC 2119 cell lines (canine MSC). Figure shows the increased rate of the dissociation of CMSC 2119 cells incubated in Cellsplit (blue) In comparison to its competitors TrypLE (green), Trypsin (red). Data shown as means (two-way ANOVA test) **p ≤ 0.01, ****p ≤ 0.0001. 

    Figure 1. Effect of different dissociation procedures on cell viability.

    Cells were expanded at 37°C for 2 weeks and retrieved when cells reached 60%-70% confluency using CellSplit® and competitor for further comparison. Detached cells were stained with Tryban Blue and CytoSMART was assessed cell viability. (A) Cell viability of HEK-F and Hep-G2 did not change when CellSplit® was used. (B) Cell viability of HEK-F decreased 4% when cells were incubated with the competitor over 5 minutes. The data is expressed as mean ± SD of technical triplicates (n=3).

    Figure 2. The impact of cell harvesting procedures on releasing cytochrome c.

    The release of cytochrome c from mitochondria is a central event in apoptosis signaling that was quantified by ELISA. Number of cells recovered by each cell dissociation reagent at 5 minutes. Some cells were treated with actinomycin-D to induce apoptotic as a positive control. (A) The release of cytochrome c protein in HEK-F cells was lower in CellSplit®. (B) The level of releasing cytochrome c in CellSplit® showed low level expression in Hep-G2 cells. The data expressed as mean ± SD of technical triplicates (n=3).

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