Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Human Platelet Lysate (hPL) is a cell culture growth supplement obtained from human platelets. hPL contains abundant human growth factors including PDGF, bFGF, EGF, TGF-beta1, VEGF, other cytokines and proteins which yield superior results when culturing many types of human cells, particularly human MSC, regardless of their tissue source. Where serum (traditionally FBS or Human)
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
hPL is derived from human donor platelets collected from healthy consented volunteer donors at certified German Blood banks (21CFR640). All individual donors and complete batches are fully tested for pathogens according to 21CFR610.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Performance: The future of cell therapy is to be xenogeneic-free and therefore human platelet lysate offers xenogeneic free/serum substitutes for replacing FBS and improving cell culture performance. Cost: FBS is commonly used at concentration between 10-20% in cell culture. However due to the high performance effects of human platelet lysate, it is typically used at
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Research Grade material is manufactured to meet the needs of basic research and development. This material supports the in vitro propagation and maintenance of various human cell types. In addition, it provides a cost effective alternative to using serum-free media. Material is supplied in validated PETG NALEGENE bottles (25 mL, 100 mL and 500 mL).
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Only the Human Platelet Lysate Standard product requires the addition of heparin. There are two types of heparin available: Heparin standard – porcine source Heparin xeno-free – fully synthetic and preservative-free
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Our ‘Xeno-Free’ products are non-xenogeneic and animal serum-free. i.e. they do not contain material from species other than human origin. Materials that are described as being ‘Xenogenic’ are derived from a different species and therefore are not genetically or immunologically compatible. This is very important for cell therapy and clinical applications.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
For both Human Platelet Lysate-XF (Xeno-Free) and Human Platelet Lysate-FD (Fibrinogen Depleted), a fibrinogen depletion step is obtained during the manufacturing process in order to remove all fibrins. In addition, heparins are added during the manufacturing process. This means that you do not need to add Heparin while preparing the cell culture mix. For Human
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Heparin is an anti-coagulant that prevents the fibrinogen in human platelet lysate from clotting in the cell culture medium during cell expansion. Both Human Platelet Lysate XF and Human Platelet Lysate FD are fibrinogen-depleted and so do not require the addition of heparin in the cell culture media.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Human Mesenchymal Stromal Cells from Bone Marrow (hMSC-BM) Human Mesenchymal Stromal Cells from Umbilical Cord (hMSC-UC) Human Mesenchymal Stromal Cells from Adipose Tissue (hMSC-AT) Human Dermal Fibroblasts (HDF) In addition, see FAQ ‘Which cell types has hPL been shown to work in?’, for a more detailed table of other cell types that hPL has been
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
No. hPL is for in vitro experimental and research use only (RUO) and the product is not intended for human or animal diagnostic or therapeutic uses.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Each batch of hPL is tested to ensure freedom from bacteria, fungi, yeast, mycoplasma (M. fermentans, M. arginini, M. orale, M. hyorhinis, M. salivarium, M. hominis, M. pneumoniae, Acholeplasma laidlawii, M. synoviae and Ureaplasma species). Batches are also tested for the ability to support growth of specific cell lines. In addition, each batch is also
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
The raw materials that go into making all our hPL products are tested and found non-reactive for HBsAg, HIV I/II antibody, CMV, HCV- and HBC-Antibody, HIV-, HCV-, HBV-, HAV-, PVB19-NAT, Treponema pallidum and Syphilis.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
hPL is aseptically processed. Each batch of hPL is tested for the absence of bacteria, fungi, yeast, and mycoplasma. Batches of hPL are also sterile filtered through three sequential 0.2 and finally 0.1 micron pore-size rated filters. Results of microbe testing of each batch of serum are supplied in the COA. However, please note that
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Human Platelet Lysate Standard – requires the addition of heparin prior to use. Choose from a standard animal derived heparin (porcine), or a xeno-free heparin (fully synthetic and preservative free). Human Platelet Lysate FD (Fibrinogen Depleted) – A fibrin-depleted hPL that does not require the addition of heparin prior to use. However, it may contain
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Research Grade: 25 mL, 100 mL and 500 mL NALGENE bottles. GMP Grade: 100 mL cryo-bags and 100 mL NALGENE bottles (standard) and 500 mL NALGENE bottles (upon request).
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Yes. Samples of hPL are available for testing prior to selection of a suitable batch. Typical sample size is 25 mL and reservations are held for a period of four weeks, pending evaluation.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
hPL has a shelf life of 24 months from the date of manufacture, provided they are stored appropriately. We would recommend enquiring about the shelf life of each available batch if it is important to have a long shelf life following purchase.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
The recommended storage is -20°C or below. hPL products can be storage long term at -80°C. Thaw frozen hPL product in a 37°C water bath before use. Once hPL product is thawed, it is recommended to fully use for completed medium preparation (e.g. 5%) within one week when stored at 4°C or to divide the
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Despite all testing, proper safety precautions for potentially infectious agents must be taken. All human blood products should be handled in accordance with currently acceptable bio-safety practices and guidelines for the prevention of blood borne viral infections.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
hPL will be shipped frozen, by overnight courier in polystyrene moulded boxes with dry ice. This will ensure that the material arrives frozen in good condition.