Monday, 08 April 2019 / Published in Human Platelet Lysate
*does not contain any heparins (these are added at the point of use). **contains residues of porcine-derived heparin. ᵻ contains residues of fully synthetic heparin. Product Key Benefits Pack sizes available Fibrinogen-depleted Requires heparin? Animal-component free Human Platelet Lysate Solution Animal serum-free; most cost effective option 25 mL, 100 mL & 500 mL No No
Friday, 05 April 2019 / Published in Human Platelet Lysate
hPL is derived from human donor platelets collected from healthy consented volunteer donors at certified blood banks. All individual donors and complete batches are fully tested for pathogens according to 21CFR610.
Friday, 05 April 2019 / Published in Human Platelet Lysate
Both Heparin and Xeno-Free Heparin are shipped at ambient temperature. Store at +4°C on arrival.
Friday, 05 April 2019 / Published in Human Platelet Lysate
All of our hPL products are derived from human platelets and therefore are naturally free form other animal derived material. No animal serum is added during the manufacture of these products. For a product to be animal-component free, no products can be added to the product during the manufacturing process that are derived from another
Friday, 05 April 2019 / Published in Human Platelet Lysate
Human Platelet Lysate (hPL) can be used at a variety of concentrations, from 10% in basal media to as little as 2.5%.  The table below indicates the concentrations of hPL used for a variety of different cell types and applications.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Human Platelet Lysate (hPL) is a cell culture growth supplement obtained from human platelets. hPL contains abundant human growth factors including PDGF, bFGF, EGF, TGF-beta1, VEGF, other cytokines and proteins which yield superior results when culturing many types of human cells, particularly human MSC, regardless of their tissue source. Serum (traditionally FBS or Human) is
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Performance: The future of cell therapy is to be xenogeneic-free and therefore human platelet lysate offers xenogeneic free/serum substitutes for replacing FBS and improving cell culture performance. Cost: FBS is commonly used at concentration between 10-20% in cell culture. However due to the high performance effects of human platelet lysate, it is typically used at
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Research Grade material is manufactured to meet the needs of basic research and development. This material supports the in vitro propagation and maintenance of various human cell types. In addition, it provides a cost effective alternative to using serum-free media. Material is supplied in validated PETG NALEGENE bottles (25 mL, 100 mL and 500 mL).
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Only the Human Platelet Lysate Standard and Customized products require the addition of heparin. There are two types of heparin available: Heparin – Standard animal-derived heparin – PLA-001M. Xeno-Free Heparin – Animal component-free heparin, fully synthetic and preservative-free – PLA-XF-001M.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Our ‘Xeno-Free’ products are non-xenogeneic and animal serum-free. i.e. they do not contain material from species other than human origin. Materials that are described as being ‘Xenogenic’ are derived from a different species and therefore are not genetically or immunologically compatible. This is very important for cell therapy and clinical applications.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
For both Human Platelet Lysate-XF (Xeno-Free) and Human Platelet Lysate-FD (Fibrinogen Depleted), a fibrinogen depletion step is obtained during the manufacturing process in order to remove all fibrins. In addition, heparins are added during the manufacturing process. This means that you do not need to add Heparin while preparing the cell culture mix. For Human
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Heparin is an anti-coagulant that prevents the fibrinogen in human platelet lysate from clotting in the cell culture medium during cell expansion. Both Human Platelet Lysate XF and Human Platelet Lysate FD are fibrinogen-depleted and so do not require the addition of heparin in the cell culture media.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Human Mesenchymal Stromal Cells from Bone Marrow (hMSC-BM) Human Mesenchymal Stromal Cells from Umbilical Cord (hMSC-UC) Human Mesenchymal Stromal Cells from Adipose Tissue (hMSC-AT) Human Dermal Fibroblasts (HDF) In addition, see FAQ ‘Which cell types has hPL been shown to work in?’, for a more detailed table of other cell types that hPL has been
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
No. hPL is for in vitro experimental and research use only (RUO) and the product is not intended for human or animal diagnostic or therapeutic uses.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Each batch of hPL is tested to ensure the absence of bacteria, fungi, yeast, mycoplasma (M. fermentans, M. arginini, M. orale, M. hyorhinis, M. salivarium, M. hominis, M. pneumoniae, Acholeplasma laidlawii, M. synoviae and Ureaplasma species). Batches are also tested for the ability to support growth of specific cell lines. In addition, each batch is
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Our hPL products are manufactured from platelet units obtained from healthy blood donors who have been tested and found negative for HBsAg, HIV I/II antibody, CMV, HCV- and HBC-Antibody, HIV-, HCV-, HBV-, HAV-, PVB19-NAT, Treponema pallidum and Syphilis.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
hPL is aseptically processed. Each batch of hPL is tested for the absence of bacteria, fungi, yeast, and mycoplasma. Batches of hPL are also sterile filtered. Results of microbe testing of each batch are supplied in the COA. However, please note that sterility is not guaranteed after opening. The use of aseptic techniques during handling
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Human Platelet Lysate Standard – requires the addition of heparin to the final cell culture medium. Choose from a standard animal derived heparin (porcine), or a xeno-free heparin (fully synthetic and preservative free). For human ex-vivo tissue and cell culture processing applications. Human Platelet Lysate FD (Fibrinogen Depleted) – A fibrinogen-depleted hPL that does not
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Research Grade: 25 mL, 100 mL and 500 mL NALGENE bottles. GMP Grade: 100 mL cryo-bags and 100 mL NALGENE bottles and 500 mL NALGENE bottles. GMP-Clinical Grade (gamma irradiated): 50 mL, 100 mL and 500 mL NALGENE bottles.
Wednesday, 10 January 2018 / Published in Human Platelet Lysate
Yes. Samples of hPL are available for testing prior to selection of a suitable batch. Typical sample size is 25 mL and reservations are held for a period of four weeks, pending evaluation.