Wednesday, 10 January 2018 / Published in FBS
Serum is the liquid fraction of clotted whole blood. Unlike plasma, no anti-coagulant will have been added to the blood following collection from the animal. Serum is centrifuged to separate the clot from the liquid phase, resulting in a product that is depleted of cells, fibrin and clotting factors.
Wednesday, 10 January 2018 / Published in FBS
Bovine serum is categorised based on the age of the animals when a serum collection is made. The age of the animal is an important consideration because it impacts on the characteristics of the serum: Foetal bovine serum comes from foetuses Newborn calf serum comes from calves less than three weeks old Calf serum comes
Wednesday, 10 January 2018 / Published in FBS
Additional sources of serum are available and are generally more cost effective options. These could also be described as FBS alternatives and can be used where budget restrictions dictate the use of cheaper material, or where applications permit the use of serum where levels of antibody and endotoxin will be higher. Alternatives include: Newborn calf
Wednesday, 10 January 2018 / Published in FBS
USDA Grade FBS is FBS of non U.S.A. origin that the United States Department of Agriculture (USDA) considers to be acceptable for importation in the US.A. These countries are defined as being free of bovine spongiform encephalopathy (BSE) and foot and mouth disease (FMD). Countries include New Zealand, Australia, Mexico and other Central American countries.
Wednesday, 10 January 2018 / Published in FBS
The country of origin for FBS refers to country in which the raw blood was collected. Major FBS producing countries are Australia, Canada, Central America, New Zealand, South America and the U.S.A. While some countries may be considered more desirable sources of FBS than others, it is not true that FBS is of better quality
Wednesday, 10 January 2018 / Published in FBS
LSP states the origin of each batch of FBS on the product label. Furthermore, this same batch information is also provided on the Certificate of Analysis (COA). As an ISIA Traceability certified company, LSP can trace raw materials back to the original supplier and abattoir or donor farm where the material was collected. This provides
Wednesday, 10 January 2018 / Published in FBS
Wide-ranging regulations control all aspects of the production of serum and other animal derived products and their subsequent use. The European Union and the United States are major users of these materials with Japan, India and China emerging as major players. The main cattle producing countries are the United States and Canada, Australia and New
Wednesday, 10 January 2018 / Published in FBS
The ISIA (International Serum Industry Association, www.serumindustry.org) has established industry standards and a certification program with the aim of helping sera users substantiate the integrity of supply of FBS and other animal sera. Major companies that collect and sell FBS globally (including LSP) are members of the ISIA. The ISIA’s mission is to ‘establish, promote
Wednesday, 10 January 2018 / Published in FBS
Certification demonstrates full compliance with ISIA traceability standards, following an independent audit. As an ISIA Traceability Certified company, LSP processes establish traceability through the entire supply chain, from raw material collection to processing, filtration and order fulfilment. According to the ISIA traceability program, Certificates of Origin or Analysis must reflect all origins present (if in
Wednesday, 10 January 2018 / Published in FBS
Each batch of LSP FBS is tested to ensure freedom from bacteria, fungi, yeast, mycoplasma (M. bovis, M. arginini and A. laidlawii) and specific bovine viruses. Batches are also tested for the ability to support growth of specific cell lines. In addition, each batch is also tested for standard parameters. These include endotoxin, pH, osmolality,
Wednesday, 10 January 2018 / Published in FBS
All LSP FBS is tested for Bovine Viral Diarrhoea Virus (BVD-V), Bovine Parainfluenza-3 virus (PI3), Infectious Bovine Rhinotracheitis (IBR) and Bovine Viral Diarrhoea Virus antibodies (BVD-AB).
Wednesday, 10 January 2018 / Published in FBS
Each batch of LSP FBS is tested for the absence of bacteria, fungi, yeast, mycoplasma. LSP FBS is also sterile filtered through three sequential 100 nm (0.1 µm) pore size-rated filters. Results of microbe testing of each batch of serum is supplied in the COA. However, please note that sterility is not guaranteed after opening.
Wednesday, 10 January 2018 / Published in FBS
The quality of FBS is inherently linked to the collection and processing methods used and not the country of origin. A closed-system collection method (cardiac or venepuncture) and rapid processing are essential to produce a quality product. Low levels of endotoxin and haemoglobin are excellent indicators of the care with which collection and processing have
Wednesday, 10 January 2018 / Published in FBS
Yes. FBS is available as heat inactivated, gamma irradiated, dialysed and charcoal stripped material.
Wednesday, 10 January 2018 / Published in FBS
FBS may be heat inactivated by heating to 56°C for 30 minutes to inactivate various components in the serum such as complement factors.
Wednesday, 10 January 2018 / Published in FBS
At one time, heat inactivation was considered necessary because of concerns over the presence of contaminants in serum. However, the process of heat inactivation is known to degrade valuable biomolecules, such as growth factors, vitamins, and amino acids. Many protocols still call for serum to be subjected to heat treatment prior to use without consideration
Wednesday, 10 January 2018 / Published in FBS
FBS is supplied in 500 mL bottles. Other pack sizes or presentations are also available on request e.g. 100 mL and 1 L bottles, or individual supplier packs.
Wednesday, 10 January 2018 / Published in FBS
Yes. LSP offer samples of FBS for testing prior to selection of a suitable batch. Typical sample size is 50 mL and reservations are held for a period of four weeks, pending evaluation.
Wednesday, 10 January 2018 / Published in FBS
FBS has a shelf life of 5 years from the date of manufacture, provided it is stored appropriately. We would recommend enquiring about the shelf life of each available batch if it is important to have a long shelf life following purchase. While there may be a drop off in growth promotion properties over time,
Wednesday, 10 January 2018 / Published in FBS
Recommended storage is -20°C or below. Protect serum from exposure to light. It is recommended to avoid freeze-thaw cycles as this can lead to a deterioration in serum qualities. Ideally, material should be thawed under controlled conditions and re-aliquoted into smaller volumes before re-freezing. It is not recommended to store or refreeze partially used serum