Caspase 3 (CASP3) BioAssay ELISA Kit (Human)

£960.00 96 Tests

Caspase 3 (CASP3) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CASP3 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US023967 Categories: ,

Additional information

Detection Range

0.156-10ng/ml

Sensitivity

0.061ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*023967A: Microtiter Strips, 1×48 wells
*023967B: Standard, 2×1 vial
023967C: Standard Diluent, 1x10ml
*023967D: Detection Reagent A (green), 1x60ul
*023967E: Detection Reagent B (red), 1x60ul
023967F: Assay Diluent A, 1x6ml
023967G: Assay Diluent B, 1x6ml
023967H: TMB Substrate, 1×4.5ml
023967K: Stop Solution, 1x3ml
023967L: Wash Buffer, 30X, 1x10ml

Precaution

The Stop Solution (023967K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023967A, *023967B, *023967D and *023967E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Specificity

Human

Shelf Life

6 Months