Cardiotrophin 1 (CT1) BioAssay ELISA Kit (Rat)

£1,007.00 96 Tests

Cardiotrophin 1 (CT1) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CT1 in rat serum, plasma and other biological fluids.

SKU: US023936 Categories: , ,

Additional information

Detection Range

15.6-1000pg/ml

Sensitivity

~5pg/ml

Precision

Intra-Assay:
CV<10%

Inter-Assay:
CV<12%

Kit Components

*023936A: Microtiter Plate, Pre-coated, 1×96 wells
*023936B: Standard (lyophilized), 2×1 vial
023936C: Standard Diluent, 1x20ml
*023936D: Detection Reagent A, 100x, 1x120ul
*023936E: Detection Reagent B, 100x, 1x120ul
023936F: Assay Diluent A, 2x, 1x6ml
023936G: Assay Diluent B, 2x, 1x6ml
023936H: TMB Substrate, 1x9ml
*023936J: Positive Control, 99.3pg/ml. 1×1 vial
023936K: Stop Solution, 1x6ml
023936L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (023936K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023936A, *023936B, *023936D, *023936E and *023936J at -20?C. After reconstitution of *023936J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Add 100ul working Detection Reagent A immediately. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul working Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Rat

Specificity

Rat

Shelf Life

6 Months