Carbonic Anhydrase II (CA2) BioAssay ELISA Kit (Human)

£901.00 96 Tests

Carbonic Anhydrase II (CA2) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CA2 in human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

SKU: US023901 Categories: , ,

Additional information

Detection Range

3.12-200ng/ml

Sensitivity

1.34ng/ml

Precision

Intra-Assay:
CV≤10%

Inter-Assay:
CV≤12%

Kit Components

*023901A: Microtiter Plate, 1x96wells, Pre-coated, ready to use
*023901B: Standard, 2x1vial
023901C: Standard Diluent, 1x20ml
*023901D: Detection Reagent A, 1x120ul
*023901E: Detection Reagent B, 1x120ul
023901F: Assay Diluent A, 2x, 1x6ml
023901G: Assay Diluent B, 2x, 1x6ml
023901H: TMB Substrate, 1x9ml
*023901J: Positive Control, 18.92ng/ml, 1x1vial
023901K: Stop Solution, 1x6ml
023901L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (023594K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023901A, *023901B, *023901D, *023901E and 023901J at -20?C. Store other components at 4?C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Specificity

Human

Shelf Life

6 Months