Calcitonin (CT) BioAssay ELISA Kit (Mouse)

£976.00 96 Tests

Calcitonin (CT) BioAssay ELISA Kit (Mouse) is a competitive inhibition immunoassay for in vitro quantitative measurement of CT in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US023846 Categories: , ,

Additional information

Detection Range

12.35-1000pg/ml

Sensitivity

4.86pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*023846A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*023846B: Standard, 2×1 vial
023846C: Standard Diluent, 1x20ml
*023846D: Detection Reagent A, 1x120ul
*023846E: Detection Reagent B, 1x120ul
023846F: Assay Diluent A, 1x12ml
023846G: Assay Diluent B, 1x12ml
023846H: TMB Substrate, 1x9ml
*023846J: Positive Control, 278.5g/ml
023846K: Stop Solution, 1x6ml
023846L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (023846K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023846A, *023846B, *023846D, *023846E and *023846J at -20?C. After reconstitution of *023846J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap

Notes on 023846J

Positive Control

Intended Use

The control vials are from a mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vials contain mouse CT in mouse serum (lyophilized powder). Note: The mouse based serum used in this product is from a healthy specimen(s) of SPF standard (specific pathogen-free animals).

Expected Value

278.5pg/ml

Usage

Dissolve with 150ul standard diluent and let stand for 10min. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Mouse

Specificity

Mouse

Shelf Life

6 Months