C-Reactive Protein (CRP) BioAssay ELISA Kit (Human)

£812.00 96 Tests

The Human C-Reactive Protein (CRP) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CRP in human serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernatants and other biological fluids.

SKU: US023797 Categories: ,

Additional information

Detection Range

62.5-4000pg/ml

Sensitivity

23.4pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*023797A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*023797B: Standard, 2×1 vial
023797C: Standard Diluent, 1x20ml
*023797D: Detection Reagent A, 1x120ul
*023797E: Detection Reagent B, 1x120ul
023797F: Assay Diluent A, 1x12ml
023797G: Assay Diluent B, 1x12ml
023797H: TMB Substrate, 1x9ml
023797K: Stop Solution, 1x6ml
023797L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (023797K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023797A, *023797B, *023797D and *023797E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Shelf Life

6 Months