BRC4wt

£135.00 1mg

Blocks BRCA2 RAD51 interaction.

BRC4wt is a peptide derived from the BRC4 repeat motif of the BReast CAncer gene 2 (BRCA2) protein, which is able to prevent the DNA repair protein RAD51 binding to BRCA2. Cancer cells rely on efficient DNA repair to survive and proliferate and the BRCA2 tumour suppressor protein is indispensable for repair of DNA double-strand breaks through promotion of RAD51 loading onto single-stranded DNA. Through blockade of this interaction BRC4wt inhibits camptothecin and ionising radiation induced RAD51 foci formation and homologous recombination in cancer cells. BRC4wt can impair the replication fork-protective function of BRCA2 and trigger double-strand break repair protein MRE11 dependent degradation of nascent DNA in response to replication stress. A ?BRC4wt cell penetrating peptide derivative confers selective hypersensitivity to PARP inhibition in cancer cells but spares noncancerous cells, giving it potential as a radio and chemosensitiser.

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Additional information

Other Names

BRC4 peptide

Three Letter Sequence

Ac-Leu-Leu-Gly-Phe-His-Thr-Ala-Ser-Gly-Lys-Lys-Val-Lys-Ile-Ala-Lys-NH2

Molecular Weight

1738.06

Molecular Formula

C81H139N23O19

Sequence

Ac-LLGFHTASGKKVKIAK-NH2

Solubility

Soluble in water

Appearance

Freeze dried solid

Storage

Store dry, frozen and in the dark

Purity

>95% by HPLC

Modifications

N terminal acetyl, C terminal amide

Searchable Words

BRC4wt, Ac-LLGFHTASGKKVKIAK-NH2, BRC4 peptide, PP-390, PP390