Brain Derived Neurotrophic Factor (BDNF) BioAssay ELISA Kit

£1,314.00 2 Plates

Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. Kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. With the BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin, enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration, i.e., the higher the OD the higher the BDNF concentration in the sample.

Additional information

Sensitivity

7.8pg/ml

Range of Detection

7.8-500pg/ml

Crossreactivity

No significant crossreactivity with NGF, NT4/5 or NT3

Intra-Assay Variation

? 3.7% (125pg/ml)

Inter-Assay Variation

? 8.5% (125pg/ml)

Kit Components

There are enough reagents included in this kit for two 96-well immuno-assay plates.
B2700-08A: BDNF ELISA Plates, 2×1 plate Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Microtiter plates.
B2700-08B: Wash Buffer, 10X, 1x100ml
B2700-08C: Standard/Sample Diluent, 1x60ml
*B2700-08D: BDNF Standard (Recombinant Human), 2×1 vials (Lyophilized).
B2700-08E: Mab (Biotin), 1x25ul
B2700-08F: Streptavidin (HRP), 1x50ul
B2700-08G: TMB/E Solution, 2x10ml
B2700-08H: Stop Solution (HCl), 1x22ml

Storage and Stability

Store at 4°C. Do not freeze. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
*B2700-08D: BDNF Standard, Store at 4°C for up to 30 days (liquid).

Shipping Temperature

On Dry Ice

Shelf Life

6 Months