Brain Derived Neurotrophic Factor (BDNF) BioAssay ELISA Kit

£1,314.00 2 Plates

Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. Kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. With the BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin, enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration, i.e., the higher the OD the higher the BDNF concentration in the sample.

Additional information



Range of Detection



No significant crossreactivity with NGF, NT4/5 or NT3

Intra-Assay Variation

? 3.7% (125pg/ml)

Inter-Assay Variation

? 8.5% (125pg/ml)

Kit Components

There are enough reagents included in this kit for two 96-well immuno-assay plates.
B2700-08A: BDNF ELISA Plates, 2×1 plate Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Microtiter plates.
B2700-08B: Wash Buffer, 10X, 1x100ml
B2700-08C: Standard/Sample Diluent, 1x60ml
*B2700-08D: BDNF Standard (Recombinant Human), 2×1 vials (Lyophilized).
B2700-08E: Mab (Biotin), 1x25ul
B2700-08F: Streptavidin (HRP), 1x50ul
B2700-08G: TMB/E Solution, 2x10ml
B2700-08H: Stop Solution (HCl), 1x22ml

Storage and Stability

Store at 4°C. Do not freeze. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
*B2700-08D: BDNF Standard, Store at 4°C for up to 30 days (liquid).

Shipping Temperature

On Dry Ice

Shelf Life

6 Months