Bone Sialoprotein (BSP) BioAssay ELISA Kit (Human)

£990.00 96 Tests

Bone Sialoprotein (BSP) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BSP in human serum, plasma, tissue homogenates and other biological fluids.

SKU: US023755 Categories: , ,

Additional information

Detection Range

125-8000pg/ml

Sensitivity

53pg/ml

Precision

Intra-Assay:
CV<10%

Inter-Assay:
CV<12%

Kit Components

*023755A: Microtiter Strips, 1×48 wells or 1×96 wells
*023755B: Standard, 2x1vial
023755C: Standard Diluent, 1x10ml or 1x20ml
*023755D: Detection Reagent A (green), 1x60ul or x120ul
*023755E: Detection Reagent B (red), 1x60ul or 1x120ul
023755F: Assay Diluent A, 1x6ml or 1x12ml
023755G: Assay Diluent B, 1x6ml or 1x12ml
023755H: TMB Substrate, 1×4.5ml or 1x9ml
*023755J: Positive Control, 4927.1pg/ml, 1x1vial
023755K: Stop Solution, 1x3ml or 1x6ml
023755L: Wash Buffer, 30x, 1x10ml or 1x20ml

Precaution

The Stop Solution (023755K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023755A, *023755B, *023755D, *023755E and *023755J at -20?C. Store all the other components at 4?C. After reconstitution of *023755J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 023755J

Positive Control

Intended Use

The control vial is from a human serum sample known to contain BSP. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vial contains human BSP in humanserum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

Expected Values

4927.1ng/ml

Usage

Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens.
Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months