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Blocking serum

Immunohistochemistry (IHC) experiments often contain one or more blocking steps to reduce background signal and false positives. Blocking with sera or a protein blocking agent is essential to prevent non-specific binding of antibodies to tissue or to Fc receptors. Serum is a common blocking agent as it contains antibodies that bind to non-specific sites. The Blocking sera should be from the same species from which the secondary antibody was raised.

Blocking serum
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