Beta-Chorionic Gonadotropin, Free, Human (hCGbeta) BioAssay ELISA Kit

£698.00 1 Kit

Principle of the Test:
The free beta-hCG ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the free beta-hCG. Mouse monoclonal anti-free-beta-hCG antibody is used for solid phase immobilization (on the microtiter wells). A goat anti-whole hCG antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the free beta-hCG molecules being sandwiched between the solid phase and enzyme-linked antibodies. After two separate 30 minute incubations at 37°C, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue colour. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of beta-hCG is directly proportional to the colour intensity of the test sample. Absorbance is measured spectrophotometrically at 450nm.

SKU: USC5069-68 Categories: ,

Additional information

Intended Use

For the quantitative determination of free beta subunit of human chorionic gonadotropin (free Beta-hCG) concentration in human serum.

Standard Size

96 wells

Range

0-50ng/ml

Sensitivity

2.5ng/ml

Crossreactivity

This kit has been validated for use with human serum samples only.

Kit Components

C5069-68A: Microtiter Plate, 1x96wells Coated with murine monoclonal anti-free beta-hCG antibody
C5069-68B: Calibrator 0ng/ml, 1x1vial
C5069-68C: Calibrator 2.5ng/ml, 1x1vial
C5069-68D: Calibrator 5ng/ml, 1x1vial
C5069-68E: Calibrator 10ng/ml, 1x1vial
C5069-68F: Calibrator 25ng/ml, 1x1vial C5069-68G: Calibrator 50ng/ml, 1x1vial C5069-68H: Sample Diluent, 1x13ml
C5069-68J: Pab (HRP), 1x18ml
C5069-68K: TMB Solution, 1x11ml
C5069-68L: Stop Solution, 1N HCl, 1x11ml

Storage and Stability

Store kit components at 4°C. Unopened kit components are stable for 6 months at 4°C. Reconstituted standards are stable for up to 30 days when stored sealed at 4°C.

Shipping Temperature

On Dry Ice

Source Antigen

Human

Shelf Life

6 Months