Tuesday, 23 June 2020 / Published in News
Life Science Group Ltd has been awarded £50,000 to complete research into a process that “inactivates the virus” immediately after swab samples are taken from patients. Read the full article here.
Wednesday, 17 June 2020 / Published in Human AB Serum
Although it is impossible to claim stability data for every possible application and use, for human serum AB we conservatively estimate that if this product is stored properly at -20ºC, it can be used up to 5 years from the date of manufacture without any decrease in product performance. Storage at any other temperature may
Wednesday, 17 June 2020 / Published in FBS, News
Endotoxins levels in serum for cell culture are important for pharmaceutical, veterinary and clinical manufacture and also in some research applications where cells are sensitive to the presence of endotoxins. It is important that serum, specifically Foetal Bovine serum (FBS), is collected in such a way to ensure that the serum maintains low levels of
Thursday, 11 June 2020 / Published in Petaka
• Class VI medical grade resin• Optically clear polystyrene growth surface• Sterile (SAL 10-6)• Standard SBS footprint• 0.2 μm PTFE air filter• 24 m filling volume• 95 kPa tested• Free of detectable endotoxin• Sterile silicone port access
Thursday, 11 June 2020 / Published in Petaka
The Petaka system is not designed for the production of tens of billions of cells. A typical suspension will produce 2.0-2.5MM cells/ml at peak density in Petaka ™, or 50MM cells per Petaka. The Petaka ™ is ideal for higher quality and safer seed culture production, or for storage and transfer of samples for cryopreservation
Thursday, 11 June 2020 / Published in Petaka
Cells may be shipped at ambient temperatures using the Petaka ™. It is recommended that the Low Oxygen Units are used for this purpose. Cells should be seeded in the Petaka ™ unit 24 hours prior to shipping to allow them to acclimatise. The Low Oxygen Transfer unit is used since it is notideal to
Thursday, 11 June 2020 / Published in Petaka
Standard plates, dishes and flasks must be open to allow open CO2 gas exchange. The Petaka ™ is functionally closed and retains CO2 in cultureautomatically. All liquid transfers are performed through a closed, sterile silicone port. An additional benefit is a reduced contamination risk during processing and the closed design means that media evaporation is
Thursday, 11 June 2020 / Published in Petaka
The Petaka ™ LOT for adherent cells features a blue silicone injection port. The Petaka ™ FLAT for suspension cells features a white silicone injection port. The orange colour port on the Petaka ™ HOT is for when you want slightly higher oxygen tension in your cultures.
Thursday, 11 June 2020 / Published in Petaka
In order to use the Petaka ™ for adherent cells and have the cells adhere to both surfaces of the Petaka ™ it is necessary to seed one side, wait for the cells to adhere for up to 3 hours, depending on the cell type and then re-seed the Petaka with cells and flip the
Thursday, 11 June 2020 / Published in Petaka
The Petaka ™ is the only cell culture device that can be centrifuged directly. This allows the user to pellet, wash and separate cells without any transfers into conical tubes. Unlike open dishes, plates, and flasks, the Petaka ™ is designed and manufactured to withstand up to 650 g during routinecentrifugation. Therefore, instead of aspirating
Thursday, 11 June 2020 / Published in Petaka
The Petaka ™ is unique in that it is possible to measure the dissolved oxygen (pO2) in your culture in real time, using a sterile, off-the-shelf setup. The Petaka ™ DO Sense features an oxygen sensing dot manufactured by Ocean Insights, that when paired with the NeoFox™ reader, allows the userto measure and record the
Thursday, 11 June 2020 / Published in Petaka
The Petaka ™ is the only cell culture device that also allows for convenient and direct cryopreservation inside the primary culture device. Cryo-vials feature a low surface-to-volume ratio. This means that the operator must use a slow freezing process while the glass transition progresses from the outside to the centre of the vial. During this
Thursday, 11 June 2020 / Published in Petaka
Because the Petaka ™ provides a unique, closed, gas-controlled culture environment without significant evaporation, it acts like a mini-incubator by itself. Because of this, speciality cell culture and experimental processes may be performed for long periods of time outside of the standardCO2 incubator environment. This is impossible with plates, dishes, flasks or bags since medium
Wednesday, 10 June 2020 / Published in Petaka
Cells naturally generate CO2. However, in plates, dishes and flasks this CO2 rapidly diffuses out of the medium, so a closed CO2 incubator system is required. The Petaka ™ automatically retains CO2 at a level sufficient to maintain pH in a standard bicarbonate-buffered medium. However, aminimum number of cells in the starting culture must be
Wednesday, 10 June 2020 / Published in Petaka
The proprietary microchannel gas regulator within each Petaka ™ will reduce the dissolved oxygen from hyperoxic levels (150 mmHg pO2) to levels found in native tissues (25-50 mmHg pO2). The exact level of dissolved oxygen will vary depending on cell type, length of uninterrupted culture and medium conditions. It is important to understand that oxygen
Wednesday, 10 June 2020 / Published in Petaka
The restrictive, respiratory microchannel in the Petaka ™ provides physiological levels of O2 while simultaneously maintaining ideal CO2 and pH levels for your culture. However, this delicate balance of inward and outward gas diffusion is not optimal when the microchannel is filled with medium. To avoid this from happening, simply keep the Petaka ™ upright
Wednesday, 10 June 2020 / Published in Petaka
One of the amazing features of the Petaka ™ is its ability to preserve cells at room temperature for an extended period of time. Many labs use the Petaka ™ to eliminate cryopreservation for processes that benefit from cell storage for up to 2-weeks. When cells are maintained at low dissolved oxygen, ideal pH levels,
Wednesday, 10 June 2020 / Published in Petaka
Suspension cells must be cultured in the Petaka ™ in a horizontal configuration similar to a flask, because these cells will clump on the bottom when the Petaka ™ is cultured upright. After adherent cells attach, (typically 1- 2 hours), the Petaka ™ may be placed in the horizontalposition or it may be switched to
Wednesday, 10 June 2020 / Published in Petaka
Practically every cell type has been successfully cultivated in the Petaka ™, including adherent, suspension, immortal, primary and transfected cells, hybridomas, MSCs, ESCs, iPSCs, etc. The Petaka ™ FLAT is untreated for suspension cell culture, while the Petaka ™ LOT is surface treatedfor adherent cells. The Petaka ™ is not just another flask; it corrects
Sunday, 29 March 2020 / Published in News
Current Covid-19 testing of patent samples follows guidelines published by the WHO [1], which have been followed by health organisations in major developed countries such as the National Health Service in the UK and [2] and CDC in the USA [3]. These protocols all transport virus ‘live’ in a cell preservation media that must be