Atto647N-LPETGG

£125.00 0.1mg

Sortase reagent.

Atto647N-LPETGG is the sortase sequence, LPETGG, coupled to the fluorescent label Atto647N. Atto647N-LPETGG can be used for site-specific labelling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. Atto647N-LPETGG contains the Atto647N fluorescent label which is efficiently excited in the range 625 – 660 nm, with strong absorption, high fluorescence quantum yield and high photo-stability.

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SKU: FP-030 Categories: ,

Additional information

Three Letter Sequence

Atto647N-Leu-Pro-Glu-Thr-Gly-Gly-OH

Molecular Weight

1200.6

Molecular Formula

C65H85N9O13

Sequence

Atto647N-LPETGG

Solubility

Soluble in DMSO

Appearance

Freeze dried solid

Storage

Store dry, frozen and in the dark

Purity

>95% by HPLC

Modifications

Atto647N at N terminal

Searchable Words

FP-030, Atto647N-LPETGG